Nurokhman, Amin and Tahani, Nadia Anisa and Faizah, Hanik and Utami, Edy Setiti Wida and Manuhara, Yosephine Sri Wulan (2018) Influence of combination of sucrose concentration and immersion frequency on biomass and flavonoid production of Gynura procumbens (Lour.) Merr Callus culture in temporary immersion bioreactor. Scholars Academic Journal of Biosciences (SAJB), 6 (12). pp. 748-754. ISSN 2347-9515; 2321-6883
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Abstract
The concentration of sucrose, in many cases could influence the production of biomass and secondary metabolite because sucrose is the main carbon source of metabolism, while liquid culture method has many advantages but has problems such as asphyxia and hyperhydricity because of long immersion. Temporary immersion bioreactor could solve problems by way of the immersion frequency. The study aimed to investigate the influence of combination sucrose concentration and immersion frequency on biomass and flavonoid production of Gynura procumbens in temporary immersion bioreactor. Callus was obtained from stem nodes and stem internodes that were grown in MS agar medium supplemented with 0.1 mg L-1 2.4-D (2.4 dichlorofenoxyaceticacid) and 0.1 mg L-1 IAA (indole acetic acid). In temporary immersion bioreactor (modification of RITA) calli were cultured in MS liquid medium supplemented with various concentration of sucrose (3%, 5%, and 7%) and immersion frequency of 15 min at 12 h interval and 5 min at 3 h interval. Cultures were maintained for 28 days. Results
showed that combination of sucrose 5% and immersion frequency of 15 min at 12 h interval produced the highest biomass, while the combination of sucrose 7% and immersion frequency of 15 min at 12 h interval produced the highest flavonoid
Item Type: | Article |
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Creators: | Creators Email NIDN Nurokhman, Amin UNSPECIFIED UNSPECIFIED Tahani, Nadia Anisa UNSPECIFIED UNSPECIFIED Faizah, Hanik hanikfaizah@uinsby.ac.id 8927000020 Utami, Edy Setiti Wida UNSPECIFIED UNSPECIFIED Manuhara, Yosephine Sri Wulan UNSPECIFIED UNSPECIFIED |
Uncontrolled Keywords: | Gynura procumbens; temporary immersion bioreactor; biomass; flavonoid; callus culture |
Subjects: | 06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060705 Plant Physiology |
Divisions: | Karya Ilmiah > Artikel |
Depositing User: | desiana ekasari |
Date Deposited: | 29 Jul 2021 15:49 |
Last Modified: | 17 Nov 2021 08:43 |
URI: | http://repository.uinsa.ac.id/id/eprint/535 |