Nurokhman, Amin and Faizah, Hanik and Sugiharto, Sugiharto and Utami, Edy Setiti Wida and Manuhara, Yosephine Sri Wulan (2019) Effect of plant growth regulator and explant types on in vitro callus induction of Gynura procumbens (Lour.) Merr. Research Journal of Biotechnology, 14 (9). pp. 102-107. ISSN 0973-6263; 2278-4535
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Abstract
Gynura procumbens (Lour.) Merr is a medicinal plant of Asteraceae. The plant is a potent compound as pharmaceutical raw material which can be increased through plant tissue culture. This study aimed to determine the effect of various combinations of plant growth regulator and explant types on induction callus. The explants used were leaf, stem node, stem internode and petiole while the plant growth regulator used were 0.1 mg/L 2,4-D and 0.1 mg/L BAP, 0.5 mg/L 2,4-D and 1.0 mg/L Kinetin, 0.5 mg/L NAA and 0.5 mg/L BAP, 5.0 mg/L 2,4 D and 0.5 mg/L BAP, 0.1 mg/L 2,4-D and 0.1 mg/L IAA. The explants were cultured in MS medium supplemented with 30% sucrose and 8% agar for 28 days. The results of this study indicated that the treatment of 0.5 mg/L NAA and 0.5 mg/L BAP on the petiole explants was the best combination of plant growth regulators to produce the highest callus fresh and dry weights (1478.1 mg and 40.0 mg respectively). Callus derived from leaf, petiole and internode explants was friable and compact in texture while node explant-derived callus was compact in texture.
Item Type: | Article |
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Creators: | Creators Email NIDN Nurokhman, Amin UNSPECIFIED UNSPECIFIED Faizah, Hanik hanikfaizah@uinsby.ac.id 8927000020 Sugiharto, Sugiharto UNSPECIFIED UNSPECIFIED Utami, Edy Setiti Wida UNSPECIFIED UNSPECIFIED Manuhara, Yosephine Sri Wulan wulanmanuhara@gmail.com UNSPECIFIED |
Uncontrolled Keywords: | Gynura procumbens; callus induction; plant growth regulator; explants type |
Subjects: | 06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060705 Plant Physiology 11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences > 111504 Pharmaceutical Sciences |
Divisions: | Karya Ilmiah > Artikel |
Depositing User: | desiana ekasari |
Date Deposited: | 30 Jul 2021 08:23 |
Last Modified: | 17 Nov 2021 07:48 |
URI: | http://repository.uinsa.ac.id/id/eprint/551 |