Arifa, Ayudya Fitri and Firdhausi, Nirmala Fitria and Hidayati, Irul and Rachmawati, Yuanita and Hadi, Moch. Irfan (2022) Optimization of PCR protocols for ITS rDNA amplification of yeasts isolated from Apis mellifera Honeycomb. Jurnal Biota, x (x). pp. 1-11. ISSN 2460-7746; 2528-262X
Yuanita Rachmawati_Optimization of PCR Protocols for ITS rDNA Amplification of Yeasts Isolated from Apis mellifera Honeycomb.pdf
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Abstract
Yeast is a microorganism that can be found in honeycomb. Yeast identification is a process to find and identify new species. One of which is molecular identification of yeast with rDNA sequences in the ITS region. Before carrying out molecular identification, it is necessary to optimize yeast DNA amplification methods to obtain good DNA sequences that ease the yeast identification process. The purpose of this study was to discover the optimum PCR (Polymerase Chain Reaction)protocols for the identification of yeasts isolated from Apis mellifera honeycomb based on the ITS rDNA. This study used 3 PCR (Polymerase Chain Reaction) protocols, i.e., from Kanti et al. (2018), Ediningsari (2008), and Maulana (2011). This results study shows that the optimum PCR protocol was from Maulana (2011), which produced clear and whole DNA fragment luminescences.
Item Type: | Article |
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Creators: | Creators Email ["eprint_fieldname_creators_NIDN" not defined] Arifa, Ayudya Fitri - - Firdhausi, Nirmala Fitria nirmala_firdhausi@yahoo.com 2025068501 Hidayati, Irul irulhidayati.alfatawi@gmail.com 198102282014032001 Rachmawati, Yuanita yuanitarhartono@gmail.com 2019088803 Hadi, Moch. Irfan m_i_h@uinsby.ac.id 0824048601 |
Uncontrolled Keywords: | Apis mellifera; PCR protocol yeast |
Subjects: | 11 MEDICAL AND HEALTH SCIENCES > 1101 Medical Biochemistry and Metabolomics > 110103 Medical Biochemistry - Inorganic Elements and Compounds 11 MEDICAL AND HEALTH SCIENCES > 1103 Clinical Sciences > 110309 Infectious Diseases |
Divisions: | Fakultas Tarbiyah dan Keguruan > Pendidikan Ilmu Pengetahuan Alam |
Depositing User: | Samidah Nurmayuni |
Date Deposited: | 24 Mar 2022 08:30 |
Last Modified: | 24 Mar 2022 08:30 |
URI: | http://repository.uinsa.ac.id/id/eprint/2238 |